The little things and monogamy


Hike:

Location:

Distance:

Trail type:

Highlights:

Valley Reserve Path

Mount Waverly, Melbourne, Australia

1.5 km

Loop trail

A bush escape in the sea of houses

Want to follow in my footsteps? Learn more about this hike here. I practise leave no trace principles. Please respect the environment and learn how to do the same on your next adventure here.


July… Winter in Melbourne brings short cold days… Covid-19 lockdown 2.0… No wonder the doom and gloom was starting to get to me. Lucky for me a short walk around the local Valley reserve in Mt Waverly brought some light back in, with the bush putting on a lovely little show. This reserve is a great oasis in the sea of houses – the beginnings of a creek, a wetland area, with some great moss, plenty of wildlife, and not many people. The short loop track, is an easy grade, with a little uneven ground on the woodland trail. I will have to come back with our greyhound April, as dogs are allowed on lead, and there are some great facilities. Already planning a picnic in the wonderful native grasslands on the next sunny Covid lock down free day. 

The nodding greenhood, Pterostylis nutans, showing off its parrot beak-like flower

What I was surprised to find on my walk was a patch of nodding greenhood orchids, Pterostylis nutans. Also known as the parrot’s beak orchid, a perfect description of the 2.5cm long, ‘nodding’ flower. Orchids have an interesting relationship with microbes. Like all Orchidaceae family members, this greenhood would have had a very close relationship with a fungus during its infancy. Colonisation by a particular fungus can be a matter of life or death of orchids. Without a fungal association at germination, the orchid will have no energy to continue growing as unlike other seeds, the orchid has virtually no energy reserves, relying on the fungal symbiont to provide its nutrition.  But even when grown, and can photosynthesises to produce its own energy, the majority of orchids are still reliant on their relationship with a fungus. These root associated fungi provide inorganic nutrition to the orchid – and orchids can supply carbon as “payment” in return for the fungal services. Many of the Australian orchid species are monogamous – having one specific fungal species associated with them. But some orchids, like the Nodding greenhood, often have some action on the side. Though P. nutans, the nodding greenhood partners up with Ceratobasidium spp there are often other fungal species associations as well. This was found back in 2007 by 3 Australian based scientists, through DNA extraction of the roots of P. nutans plants from across the east coast of Australia. 

Simtalapis- this is the recipe

I stumbled on this park as I was delivering contact-free cake to celebrate a PhD student submitting their thesis. During Covid we have been celebrating the small things as well as the larger wins such as a thesis submission. Hoping to bring a taste of home to the celebration I tried my hand at the Lithuianian, Simtalapis. Paper-fine yeast dough, layered with butter (and more butter) wrapped around ground poppy seeds with sultanas. A test in patience and willpower – grinding 400g of poppy seeds is an afternoon effort when your grinder is a hand-cranked coffee grinder that fits 20g of poppy seeds at a time. Want to make your own Simtalapis? This is the recipe I followed, however I am still not sure where all the butter goes. 

Celebrate the wins, and enjoy the small things! 

Have you looked at the trunks of trees lately? The scars tell stories of the past or hold a city of creatures in the bark.

Kulin nation scarred tree: This red gum has a distinctive shaped scar, where the bark was removed to possibly construct a canoe. Moved from its original location in Glen Waverly, due to the expansion of Springvale Rd in 1965.
Have you looked around at your feet? Greenhood orchids nodding in a bed of moss.
Have you inspected the bushes? What creatures do they hold? Hover fly dusted in pollen on the spirals of hakea flowers
Jenn masked up amongst the gums at Valley reserve
The tracks in Valley Reserve

Further reading:

Pterostylis nutans (Orchidaceae) has a specific association with two Ceratobasidium root-associated fungi across its range in eastern Australia. Mycoscience, 2007


Dr Jenn Payne
@DrPayne_AMR

Ice castles

The wanderings of a Hugh Rogers fellow through a Melbourne-Boston Sister-City association


Hike:

Location:

Distance:

Trail type:

Highlights:

Mount Moosilauke, via Gorge Brook Trail

White Mountains, NH, USA

13 km

There and back again, possible loop

Moose, stunning uninterrupted views of the white mountains,

Want to follow in my footsteps? Learn more about this hike here. I practise leave no trace principles. Please respect the environment and learn how to do the same on your next adventure here.


Jenn on the snow-covered Gorge Brook Trail on the way to the top of Mount Moosilauke.

“Do you have microspikes?” Oh no I thought, not this again. This micro has nothing to do with microbiology, and everything to do with ice. 

Last time I was asked if I had microspikes it ended in a hike involving climbing up and down frozen waterfalls. Yes, that sounds cool, and it is. But when you have developed a fear of ice it takes a little getting used to. This fear has developed while walking around Boston, as I am never entirely sure when I am going to end up on my butt from stepping on black ice. I am considering these microspikes, metal spikes that strap on to your hiking boots, might be an excellent addition for my daily walking commute to Harvard. 

Today these microspikes are for a hike up Mount Moosilauke in the White Mountains of New Hampshire. 

The trailhead is at the Dartmouth Moosilauke Ravine Lodge, a giant log cabin that welcomes anyone for something to eat or even to stay. No snacks for us here as the lodge is closed over winter. With this closure, the road in is also closed, adding a bit to your walk as you park off Ravine Road. Not to worry you might see a moose along the quiet road. Unfortunately, all we came across of these “mythical’ creatures was poo and hoof prints. 

The mythical moose foot prints

Once on the trail, you are surrounded by pine trees, a gentle bubbling creek and a gradual climb. You know you are close to the summit as the track steepens, the trees grow smaller, and glimmers of the surrounding mounts start to peek out through the trees. 

The exposed ridge to the summit is dotted with large stone cairns marking the way. Thankful for the microspikes to safely traverse the tightly compacted snow and ice, we made it to the summit without being blown away. We couldn’t enjoy the stunning 360-degree views for long as it was blowing blustering gale-force winds. Conditions common for the Moosilauke peak and unsurprising as just a hop, skip, and jump away on the top of Mt Washington has recorded the second-highest wind speed measured anywhere in the world of 372 km/h. 

Well worth the visit, the summit offers up stunning views in all directions. Being the western most of New Hampshire’s 48 peaks and the 10th highest, you have stunning vistas of the mountain ranges – on a clear day like ours we could see some of the Adirondack Mountains of New York and the Green Mountains of Vermont. But the best was the view to the west taking in Franconia Ridge. 

We had considered doing this hike as a loop, circling back to the ravine lodge via the carriage trail, but with the wind howling up that side of the mountain, and being an exposed trail, we decided the more pleasant option would be back down the protected route we had taken up.

The wind blown ice castles on the summit of Mount Moosilauke.

It wasn’t just the stunning views that captured my attention on the summit. The natural ice sculptures created by the wind, looked like built structures, making me think of ice castles. But what is cool, is that even on a microscopic level these can be considered castles. The individual snowflake is an ice fortress built for a microbe. Bacteria and dust floating in the air act as nucleation sites for the formation of snowflakes. 

A common snowflake inhabiting microbe is Pseudomonas syringae, a rod-shaped, Gram-negative bacteria. Don’t worry, no need to stop catching snowflakes on your tongue, as P. syringae is not a superbug, instead it devastates our crops. It does have a superpower though – it can turn water into ice. This super power means that is a fantastic snowflake nucleator. 

P. syringae produced its own ice castles through a protein on the bacterial surface – the outer cell membrane protein, inaZ. The surface of inaZ mimics ice, this mimicry of structure causes water molecules that come into contact with it to arrange themselves like an ice lattice. Water molecules that have been templated into this lattice formation become ice quicker. Thus, freezing at lower temperatures and becoming a snowflake quicker. It is this protein on P. syringae that means they are able to transform water vapor into ice at temperatures higher than cloud freezing, creating their snowflake ice castle. 

The inaZ protein enables them to make more snowflakes than other ice nucleators – like dust or pollen. This has made them a key component of our man-made snowfields – P. syringae is used to make Snomax, a product that enhances snowmaking, perhaps even used at your local snow resort. This bacterial protein may be a helpful factor for the future of snow play in Australia with the ever-warming winters means less snow. This little microbe may just help ensure your next skiing adventure and even that next rain. 

Mini city of fungi and lichen peeking out through the snow on Mt Moosilauke.

This adventure was made possible by a Hugh Rogers Fellowship, from the Melbourne Boston Sister City association.

Media Coverage of Jenn Payne’s Hugh Rodgers Fellowship:

Want to start your own adventure in Boston? Apply for a Hugh Rodgers Fellowship! They are not just for scientists- these fellowships support education and the arts too.


Further reading:

Ubiquity of Biological Ice Nucleators in Snowfall. Science, 2008.

Ice-nucleating bacteria control the order and dynamics of interfacial water. Science Advances, 2016.

Bacteria in the Leaf Ecosystem with Emphasis on Pseudomonas syringae—a Pathogen, Ice Nucleus, and Epiphyte. Microbiology and Molecular Biology Reviews, 2000

Snowmax.


Dr Jenn Payne
@DrPayne_AMR